FIGURE

Fig. 3

ID

ZDB-FIG-170517-11

Publication

Rao et al., 2017 - Neurotransmitter-Regulated Regeneration in the Zebrafish Retina

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Fig. 3

AMPA and Muscimol Injections Suppress Regeneration in Damaged Retinas

(A) Model illustrating effects of muscimol and AMPA injections on MG proliferation.

(B–E) Tg(zop:nfsb-EGFP)^nt19 fish were treated with 10 mM metronidazole for 24 hr, then allowed to recover. Fish were then anesthetized and injected with either AMPA at 28-hr recovery (C) or muscimol at 48-hr recovery (E). PBS controls were also anesthetized and injected at 28-hr (B) or 48-hr recovery (D). All injected eyes were removed at 52-hr recovery. Proliferation was assessed by PCNA staining. Representative images are small portions of the entire retina. Scale bar, 100 μm.

(F) Clusters of proliferating cells were measured across entire sections for PBS at 28 hr recovery (n = 9 eyes analyzed), AMPA at 28 hr recovery (n = 8), PBS at 48 hr recovery (n = 4), and muscimol at 48 hr recovery (n = 7). Two-tailed Student’s t test was used; error bars denote SD; ^∗p < 0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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