FIGURE

Fig. S5

ID

ZDB-FIG-170512-43

Publication

Keightley et al., 2017 - The Pu.1 target gene Zbtb11 regulates neutrophil development through its integrase-like HHCC zinc finger

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Fig. S5

Zbtb11 deficiency results in increased apoptosis and cell cycle arrest (supports Fig. 4)

a. Overlay of lyz:dsRed and secA5:mVenus expression shows that apoptotic cells are not coincident with neutrophils in mne. Area in yellow box is expanded in lower panel;

b. Quantification of apoptotic cells in zbtb11lo. Pooled data with embryos from individual experiments shown in different colours (mne (n=1) or zbtb11 morphants (n=2) and WT (n=3) experiments); from left to right: n=25,28,30,20; mean ±SEM; Mann-Whitney test);

c. Representative images of EdU positive cells in the caudal haemopoietic tissue in WT and mne;

d. Quantification of EdU positive cells in mne and WT; n=3 experiments, pooled data with embryos from individual experiments shown in different colours; WT, n=63; mne, n=73; mean ±SEM; Mann-Whitney test); panels a, c scale bar = 200 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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