FIGURE

Fig. 5

ID

ZDB-FIG-170321-42

Publication

Chen et al., 2017 - Neuroprotective Role of the PI3 Kinase/Akt Signaling Pathway in Zebrafish

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Fig. 5

Neuronal cell abundance, but not fate, is affected by Akt overexpression through influencing apoptosis. (A) Representative whole mount in situ hybridization images of control mRNA (left panels) or myr-Akt mRNA-injected embryos (right panels) using probes for (a) egr2b (third and fifth rhombomeres of the hindbrain), (b) emx1 (forebrain), (c) pax2a (optic stalk, mid-hindbrain boundary), and (d) rx1 (retina). N = 9–13 embryos per group, all with similar expression patterns. Scale bar = 100 µm. (B) Whole mount in situ hybridization of embryos treated with or without LY294002 using probes as (A). (C) Acridine orange staining of 24 hpf wild-type and myr-Akt mRNA-injected embryos treated without or with 8 µM, 16 µM LY294002. Scale bar = 100 μm. (D) Graphical representation of percentages of Median relative brightness of brain region after AO staining in each treatment group. N = 15 images quantified per treatment group. Groups with different letters differ significantly from each other (P < 0.05).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)