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Fig. 5
Neuronal cell abundance, but not fate, is affected by Akt overexpression through influencing apoptosis. (A) Representative whole mount in situ hybridization images of control mRNA (left panels) or myr-Akt mRNA-injected embryos (right panels) using probes for (a) egr2b (third and fifth rhombomeres of the hindbrain), (b) emx1 (forebrain), (c) pax2a (optic stalk, mid-hindbrain boundary), and (d) rx1 (retina). N = 9–13 embryos per group, all with similar expression patterns. Scale bar = 100 µm. (B) Whole mount in situ hybridization of embryos treated with or without LY294002 using probes as (A). (C) Acridine orange staining of 24 hpf wild-type and myr-Akt mRNA-injected embryos treated without or with 8 µM, 16 µM LY294002. Scale bar = 100 μm. (D) Graphical representation of percentages of Median relative brightness of brain region after AO staining in each treatment group. N = 15 images quantified per treatment group. Groups with different letters differ significantly from each other (P < 0.05).