FIGURE

Fig. 1

ID

ZDB-FIG-170321-39

Publication

Chen et al., 2017 - Neuroprotective Role of the PI3 Kinase/Akt Signaling Pathway in Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Inhibition of phosphoinositide 3-kinase leads to decrease of brain size significantly over growth retardation. (A) Representative Western immunoblots using antibodies for phosphorylated and total Akt on embryos treated with 8 μM, 16 μM, or without LY294002. Note the increase in total levels of Akt and gradually decreased phosphorylated Akt in LY294002 treated embryos compared to controls. Similar results were obtained in three other treatment experiments. (B) Morphology of 24 hpf embryos of wild type and treated with 8 µM, 16 µM LY294002. The top panel, scale bar = 500 μm; the bottom panel shows a close-up of the head region, scale bar = 200 μm. (C) Brain thickness and (D) relative heights of embryos from control group and embryos treated with 8 µM, 16 µM LY294002. Results are from three independent microinjection experiments, each with 30 embryos per group. P < 0.05 compared with the control group.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)