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Fig. 1
Inhibition of phosphoinositide 3-kinase leads to decrease of brain size significantly over growth retardation. (A) Representative Western immunoblots using antibodies for phosphorylated and total Akt on embryos treated with 8 ?M, 16 ?M, or without LY294002. Note the increase in total levels of Akt and gradually decreased phosphorylated Akt in LY294002 treated embryos compared to controls. Similar results were obtained in three other treatment experiments. (B) Morphology of 24 hpf embryos of wild type and treated with 8 µM, 16 µM LY294002. The top panel, scale bar = 500 ?m; the bottom panel shows a close-up of the head region, scale bar = 200 ?m. (C) Brain thickness and (D) relative heights of embryos from control group and embryos treated with 8 µM, 16 µM LY294002. Results are from three independent microinjection experiments, each with 30 embryos per group. P < 0.05 compared with the control group.