FIGURE

Fig. 7

ID

ZDB-FIG-170314-18

Publication

Meda et al., 2016 - Nerves Control Redox Levels in Mature Tissues Through Schwann Cells and Hedgehog Signaling

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

H₂O₂ stimulates axon growth in a positive feedback loop. (A) Scheme of the experiments. (B) ROS detection with DCFDA fluorescent probe. (C, D) Detection of H₂O₂ in ubi:HyPer fish. Fish were incubated in water (C) or Nox-i (D) and fluorescence of HyPer analyzed on adult anesthetized fish. [H₂O₂] is inferred from the YFP₅₀₀/YFP₄₂₀ excitation ratio of HyPer. Pseudocolor calibration bars: HyPer ratio (YFP₅₀₀/YFP₄₂₀). (E, F) Growing nerves were detected in the regenerating fin at 24 hpa by immunodetection of acetylated tubulin in fish incubated in vehicle (E), Nox-i (F), or HH-i (not shown). The quantification presented in (G) corresponds to the number of rays with growing nerves per half fin (maximum 9). n Values: dimethyl sulfoxide = 36; NOX-i = 29; HH-i = 11. (H) This article reveals the interaction between two feedback loops in the regulation of axon growth. Error bars represent the SEM (**p < 0.01; ***p < 0.001). Scale bars = 200 μM. YFP, yellow fluorescent protein.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Antioxid. Redox Signal.