Fig. 2
Systemic Sox10 activation in SCs following amputation. (A) Scheme of the experiment. (B–H) Caudal fin of adult fish was amputated (t = 0, blue arrow), and the axon cytoskeleton (red) and immature SCs (white) were visualized over time through immunofluorescence staining for the axonal marker, acetylated tubulin (red), and the immature SC marker, Sox10 (white), respectively. Immunostaining in uncut fin (B) or after amputation (C–H). (B–G) The upper panel shows the distal part of the amputated fin, and the lower panel shows a more proximal part. (H′) Shows a higher magnification of (H). Dotted line: amputation plane. Dashed line: distal part of the fin. Scale bars = 50 μM. R, ray; IR, inter-ray. |