FIGURE

Fig. 2 S2

ID
ZDB-FIG-170309-14
Publication
Wircer et al., 2017 - Homeodomain protein Otp affects developmental neuropeptide switching in oxytocin neurons associated with a long-term effect on social behavior
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2 S2

Expression of oxt and its receptor by PT OXT neurons.

(A) Whole-mount in situ hybridization with DIG-labeled RNA probe directed against the oxt followed by a long (e.g. to saturation) development to NBT-BCIP colorimetric substrate. The specimen was then subjected to immunostaining with an anti-tyrosine hydroxylase (TH) antibody to detect dopaminergic neurons that serves as a landmark for the position of the posterior tuberculum (PT). Arrowheads in the left and right (zoomed in) panels indicates the position of PT OXT neurons expressing low but detectable levels of oxt mRNA. Scale bar, 100 µm. (B) A representative image (single confocal plane) of a transgenic Tg(oxt:egfp) larvae, which was subjected to fluorescent in situ hybridization with an oxytocin receptor (oxtr) mRNA probe (red) followed by immunostaining for EGFP (green). PT OXT cells that express oxtr are indicated by arrowheads. Scale bar, 20 µm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife