Fig. S1

Characterisation and quantification of vessels in vegfd^uq9bh mutant embryos. (A) Quantification of the percentage of arterial intersegmental vessels (aISVs) and venous intersegmental vessels (vISVs) at 5dpf per somite across 10 somites anterior from the end of the yolk extension in vegfd^uq9bh heterozygous, mutant and wildtype embryos reveals no significant differences between genotypes. Error bars represent mean +/- sem; one way ANOVA analysis (n=6). (B) Quantification of the percentage of ISLVs at 5dpf per somite across 10 somites anterior from the end of the yolk extension in vegfd^uq9bh heterozygous, mutant and wildtype embryos reveals no significant differences between genotypes. Error bars represent mean +/- sem; one way ANOVA analysis (n=6). (C) Analysis of vegfd mRNA levels in embryos derived from an incross of vegfd adult mutants showing mutant embryos had approximately 50% of the transcript levels of wildtype embryos, suggesting non-sense mediated decay of mutant transcripts There was no change in vegfc mRNA levels in vegfd^uq9bh mutants indicating that compensation for the loss of vegfd does not occur in zebrafish. (D) Embryos from a vegfd^uq9bh heterozygote x vegfd^uq9bh homozygous mutant cross, injected with vegfc morpholino, were characterised for the loss of facial lymphatics as severe, moderate or none. (E) Subsequent genotyping found that the vegfd^uq9bh mutant embryos were enriched in the severe loss of facial lymphatic category.