FIGURE

Fig. 1

ID
ZDB-FIG-170308-2
Publication
Bower et al., 2017 - Vegfd modulates both angiogenesis and lymphangiogenesis during zebrafish embryonic development
Other Figures
All Figure Page
Back to All Figure Page
Fig. 1

vegfd regulates facial lymphangiogenesis. (A) Forward (red) and reverse (blue) Talens used to target the vegfd locus induced a 7 bp deletion designated as the uq9bh allele. (B) The uq9bh allele results in a premature stop codon at amino acid 65, prior to the VEGF homology domain. (C,D) Confocal images of Tg(-5.2lyve1b:dsRed);Tg(flt1:yfp) wild-type (C, lyve1b:dsRed only; C′) and vegfduq9bh mutant embryos (D, lyve1b:dsRed only; D′) at 5 dpf. (E-H) vegfduq9bh adult mutants are viable and have no obvious defects (G,H) when compared with siblings (E,F). (J-N′) Confocal images showing the facial region of 5 dpf Tg(fli1a:negfp);Tg(-5.2lyve1b:dsRed) transgenic embryos produced by in-crossing vegfduq9bh, vegfchu5055 double heterozygotes. Arrows indicate the presence of normal medial facial lymphatics (MFL), lateral facial lymphatics (LFL) and otolithic lymphatic vessel (OLV). Asterisks indicate the reduction of LEC number in these vessels. (O-Q) vegfduq9bh mutant embryos have a reduced number of facial lymphatic endothelial cells (LECs) when quantified in 5 dpf Tg(fli1a:negfp):Tg(-5.2lyve1b:dsRed) embryos. (O) Significant differences in LFL LEC numbers were observed when vegfduq9bh mutants (n=5) were compared with vegfduq9bh heterozygous (n=12) or wild-type (n=7) embryos in a vegfchu5055 wild-type background; and when vegfduq9bh mutants (n=7) were compared with vegfduq9bh heterozygous (n=18) or wild-type (n=6) embryos in vegfchu5055 mutants. (P) Significant differences in MFL LEC numbers were observed when comparing vegfduq9bh mutants (n=6) with vegfduq9bh wild-type (n=7) embryos in a vegfchu5055 wild-type background; when comparing vegfduq9bh mutant (n=10) or heterozygous embryos (n=18) with vegfduq9bh wild type (n=6) and embryos in a vegfchu5055 heterozygous background; and when vegfduq9bh mutants (n=9) were compared with vegfduq9bh heterozygous (n=18) and wild-type (n=6) embryos in a vegfchu5055 mutant background. (Q) There was no interaction between vegfc and vegfd in the formation of the otolithic lymphatic vessel. Data represent mean±s.e.m.; ***P<0.001, ** P<0.01, *P<0.05 from one-way ANOVA from three independent clutches of embryos.

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage: Day 5

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Day 5

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development