Contraction-dependent myofibril damage to the slow muscle of ttn.2xu064. (A) Slow muscle defects in ttn.2xu064 embryos. Immunostaining for Myosin and Phalloidin staining to label Actin filaments in 2?dpf embryos. Arrows indicate the two rod-like structures enclosing the damaged myofibril region. (B) Fast muscle is intact in ttn.2xu064. Immunostaining with antibodies against Tropomyosin and Myomesin or staining with Phalloidin in 2?dpf embryos. (C) ttn.2xu064 mutants and siblings treated with 1% tricaine for 24?h or 0.07% bleach at 1?dpf for 5?min were fixed and immunostained for Myosin at 2?dpf. Scale bars: 5??m.
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