FIGURE

Fig. 2

ID

ZDB-FIG-170123-1

Publication

Shih et al., 2016 - Exon- and contraction-dependent functions of titin in sarcomere assembly

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Exon-dependent functions of titin in sarcomere assembly in somites. (A) ttn.2^xu065, but not ttn.2^xu064, exhibits disrupted Z-disk formation in somites at 2?dpf. Shown are whole-mount immunostaining for ?-Actinin. The inset is a magnified image showing residual striated structure in ttn.2^xu065. (B) ttn.1^xu066, but not ttn.1^xu067, exhibits sarcomere disarray in somites at 9?dpf. (C) Schematic representation of the ttn.2 cis double mutant ttn.2^xu068. Arrows indicate locations of mutations. (D) Immunostaining for ?-Actinin in somites at 2?dpf indicates that the Z-disk structure in ttn.2^xu065 and ttn.2^xu068 mutants is disrupted to the same extent. Scale bars: 20??m and 8??m (inset in A).

Expression Data

Antibody:	Ab1-actn
Fish:	WT ttn.1^xu066/xu066 ttn.1^xu067/xu067 ttn.2^xu064/xu064 ttn.2^xu065/xu065 ttn.2^xu068/xu068
Anatomical Terms:	myotome sarcomere somite sarcomere somite Z disc
Stage Range:	Long-pec to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ttn.1^xu066/xu066 ttn.1^xu067/xu067 ttn.2^xu064/xu064 ttn.2^xu065/xu065 ttn.2^xu068/xu068
Observed In:	myotome sarcomere somite myofibril somite sarcomere somite Z disc
Stage Range:	Long-pec to Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development