FIGURE

Fig. 2

ID

ZDB-FIG-170123-1

Publication

Shih et al., 2016 - Exon- and contraction-dependent functions of titin in sarcomere assembly

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Fig. 2

Exon-dependent functions of titin in sarcomere assembly in somites. (A) ttn.2^xu065, but not ttn.2^xu064, exhibits disrupted Z-disk formation in somites at 2 dpf. Shown are whole-mount immunostaining for α-Actinin. The inset is a magnified image showing residual striated structure in ttn.2^xu065. (B) ttn.1^xu066, but not ttn.1^xu067, exhibits sarcomere disarray in somites at 9 dpf. (C) Schematic representation of the ttn.2 cis double mutant ttn.2^xu068. Arrows indicate locations of mutations. (D) Immunostaining for α-Actinin in somites at 2 dpf indicates that the Z-disk structure in ttn.2^xu065 and ttn.2^xu068 mutants is disrupted to the same extent. Scale bars: 20 μm and 8 μm (inset in A).

Expression Data

Antibody:	Ab1-actn
Fish:	WT ttn.1^xu066/xu066 ttn.1^xu067/xu067 ttn.2^xu064/xu064 ttn.2^xu065/xu065 ttn.2^xu068/xu068
Anatomical Terms:	myotome sarcomere somite sarcomere somite Z disc
Stage Range:	Long-pec to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ttn.1^xu066/xu066 ttn.1^xu067/xu067 ttn.2^xu064/xu064 ttn.2^xu065/xu065 ttn.2^xu068/xu068
Observed In:	myotome sarcomere somite myofibril somite sarcomere somite Z disc
Stage Range:	Long-pec to Days 7-13

Phenotype Detail

Acknowledgments

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