FIGURE

Fig. 4

ID

ZDB-FIG-170123-2

Publication

Shih et al., 2016 - Exon- and contraction-dependent functions of titin in sarcomere assembly

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Fig. 4

Mutants ttn^xu69 and ttn^xu070 overcome protein compensation and exhibit more severe phenotypes. (A) Schematic representation of two double mutants, ttn^xu069 and ttn^xu070. Arrows indicate locations of mutations. (B) Protein extracts from 2 dpf larvae of ttn^xu69 mutants analyzed by 2% SDS-agarose gel and silver staining. (C) Protein analysis of ttn^xu070. (D) ttn.2 and ttn.1 mRNA expression levels in ttn^xu070 and ttn^xu69 mutants, as revealed by qPCR using primers targeting ttn.2 and ttn.1, respectively. Means±s.d., N=5. **P<0.01. (E) Immunostaining of 2 dpf embryos for α-Actinin. Scale bar: 20 μm.

Expression Data

Genes:	ttn.1 ttn.2
Antibody:	Ab1-actn
Fish:	WT ttn.1^xu066/xu066 ttn.1^xu067/xu067 ttn.1^{xu069a/xu069a} ttn.1^{xu070a/xu070a} ttn.2^xu065/xu065
Anatomical Terms:	somite sarcomere somite Z disc whole organism
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ttn.1^{xu069a/xu069a} ttn.1^{xu070a/xu070a} ttn.2^xu065/xu065
Observed In:	somite sarcomere somite Z disc whole organism
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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