Fig. 8
Contraction-dependent myofibril damage to the slow muscle of ttn.2xu064. (A) Slow muscle defects in ttn.2xu064 embryos. Immunostaining for Myosin and Phalloidin staining to label Actin filaments in 2 dpf embryos. Arrows indicate the two rod-like structures enclosing the damaged myofibril region. (B) Fast muscle is intact in ttn.2xu064. Immunostaining with antibodies against Tropomyosin and Myomesin or staining with Phalloidin in 2 dpf embryos. (C) ttn.2xu064 mutants and siblings treated with 1% tricaine for 24 h or 0.07% bleach at 1 dpf for 5 min were fixed and immunostained for Myosin at 2 dpf. Scale bars: 5 μm.