FIGURE

Fig. 3

ID

ZDB-FIG-161209-14

Publication

Zhang et al., 2016 - Manipulating the air-filled zebrafish swim bladder as a neutrophilic inflammation model for acute lung injury

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

LPS induces inflammatory cytokine expression and injury in the zebrafish swim bladder. (a) Whole-body mRNA levels for IL-1β, IL-6, TNF-α and TNF-β increased after the 50-ng LPS injection. The results are presented as the mean±S.E.M.; n=30 in each group; *P<0.05; **P<0.01; (Student’s t-test); three independent experiments. (b) The changes to the mRNA levels for IL-1β, IL-6, TNF-α and TNF-β in the swim bladder after the 50-ng LPS injection. n=100 swim bladders per group. (c) Damaged epithelial layer in the swim bladder of LPS-injected (50 ng) zebrafish larvae at 5 dpf (indicated by black arrows). (d) Swollen ER (indicated by white arrowheads) and the control (indicated by white arrows). Mitochondrial injuries include mitochondrial cristae breakage (indicated by asterisks) and vacuolation (indicated by black arrowheads). The control mitochondria are indicated by black arrows. The lowers panels (scale bar: 0.25 μm) are magnifications of the upper panels (scale bar: 0.5 μm)

Expression Data

Genes:	il1b il6 tnfb
Fish:	i113Tg
Condition:	chemical treatment by injection: lipopolysaccharide
Anatomical Terms:	swim bladder whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	i113Tg
Condition:	chemical treatment by injection: lipopolysaccharide
Observed In:	swim bladder swim bladder endoplasmic reticulum swim bladder epithelium swim bladder mitochondrial crista swim bladder mitochondrion whole organism
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Death Dis.