FIGURE

Fig. 1

ID

ZDB-FIG-161209-12

Publication

Zhang et al., 2016 - Manipulating the air-filled zebrafish swim bladder as a neutrophilic inflammation model for acute lung injury

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Fig. 1

Neutrophils migrate into the LPS-injected zebrafish swim bladder. (a) The dose response for neutrophil recruitment into the swim bladder of 5-dpf zebrafish larvae after stimulation with LPS, Poly IC and Nano-SiO₂ for 4 hpi. (b) Neutrophil accumulation into the Tg(mpo:GFP) zebrafish (5 dpf) swim bladder after the 50 ng LPS injection was imaged by confocal microscopy at 5 hpi (maximum projection slices n=66; green, neutrophil; scale bar: 60 μm). (c) Neutrophil recruitment to the LPS-injected swim bladder (indicated by the dotted line) of Tg(mpo:GFP) zebrafish larvae at 5 dpf was monitored (green, neutrophil; scale bar: 100 μm). (d) The neutrophil distribution (green) throughout the whole body (scale bar: 100 μm). (e) HE staining of zebrafish (5 dpf) swim bladders after the LPS challenge (scale bar: 20 μm)

Expression Data

Gene:	GFP
Fish:	i113Tg
Condition:	chemical treatment by injection: lipopolysaccharide
Anatomical Terms:	blood vessel capillary neutrophil
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	i113Tg
Conditions:	chemical treatment by injection: lipopolysaccharide chemical treatment by injection: silicon dioxide nanoparticle chemical treatment by injection: poly(I:C)
Observed In:	neutrophil neutrophil chemotaxis swim bladder
Stage:	Day 5

Phenotype Detail

Acknowledgments

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