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ZFIN ID: ZDB-FIG-161205-5
Cayuso et al., 2016 - EphrinB1/EphB3b Coordinate Bidirectional Epithelial-Mesenchymal Interactions Controlling Liver Morphogenesis and Laterality. Developmental Cell   39:316-328 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 26+ somites to Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 26+ somites to Prim-15

Fig. 5

EphB3b Controls LPM Polarity and Asymmetric Movement

(A-E′) EphrinB1 and EphB3b regulate LPM polarity in the foregut region. α-ZO-1 staining in MO-ephb3b embryos reveals that junctions in the LPM form similar to controls at 22 hpf (A-B′); while at 32 hpf, ZO-1 is mislocalized (arrowheads) in MO-ephrinb1 and MO-ephb3b embryos (C-E′). Yellow arrowheads specify the midline and reveal impaired gut looping in MO-ephrinb1 and MO-ephb3b (C-E).

(F-G′) casanova mutants exhibit ZO-1 localization defects in the LPM (arrowheads) at 32 hpf.

(A-G′) Transverse sections at liver level, left side to the right; dashed lines delineate the endoderm (green) and LPM (yellow); (A′-G′) are magnifications of the areas indicated by a box in (A-G).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
efnb1 WT control 26+ somites intestinal rod IHC
26+ somites lateral plate mesoderm IHC
WT + MO1-ephb3b + MO2-ephb3b standard conditions 26+ somites intestinal rod IHC
26+ somites lateral plate mesoderm IHC
prox1a zf106Tg control Prim-15 hepatoblast IHC
zf106Tg + MO1-ephb3b + MO2-ephb3b standard conditions Prim-15 hepatoblast IHC
zf106Tg + MO2-efnb1 + MO3-efnb1 standard conditions Prim-15 hepatoblast IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tjp1 IHC WT control 26+ somites lateral plate mesoderm apical junction complex
IHC Prim-15 mesoderm apical junction complex
IHC WT + MO1-ephb3b + MO2-ephb3b standard conditions 26+ somites lateral plate mesoderm apical junction complex
IHC Prim-15 mesoderm apical junction complex
IHC WT + MO2-efnb1 + MO3-efnb1 standard conditions Prim-15 mesoderm apical junction complex
IHC sox32ta56/ta56 standard conditions Prim-15 mesoderm apical junction complex
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-ephb3b + MO2-ephb3b standard conditions 26+ somites lateral plate mesoderm establishment of epithelial cell apical/basal polarity process quality, normal
Prim-15 embryonic digestive tract development decreased process quality, abnormal
Prim-15 intestinal bulb primordium decreased curvature, abnormal
Prim-15 lateral plate mesoderm establishment of epithelial cell apical/basal polarity decreased process quality, abnormal
Prim-15 mesoderm apical junction complex ab1-tjp1 labeling mislocalised, abnormal
WT + MO2-efnb1 + MO3-efnb1 standard conditions Prim-15 embryonic digestive tract development decreased process quality, abnormal
Prim-15 intestinal bulb primordium decreased curvature, abnormal
Prim-15 lateral plate mesoderm establishment of epithelial cell apical/basal polarity decreased process quality, abnormal
Prim-15 mesoderm apical junction complex ab1-tjp1 labeling mislocalised, abnormal
sox32ta56/ta56 standard conditions Prim-15 lateral plate mesoderm establishment of epithelial cell apical/basal polarity decreased process quality, abnormal
Prim-15 mesoderm apical junction complex ab1-tjp1 labeling mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 39, Cayuso, J., Dzementsei, A., Fischer, J.C., Karemore, G., Caviglia, S., Bartholdson, J., Wright, G.J., Ober, E.A., EphrinB1/EphB3b Coordinate Bidirectional Epithelial-Mesenchymal Interactions Controlling Liver Morphogenesis and Laterality, 316-328, Copyright (2016) with permission from Elsevier. Full text @ Dev. Cell