FIGURE

Fig. S8

ID

ZDB-FIG-161110-22

Publication

Cheng et al., 2016 - Vinculin b deficiency causes epicardial hyperplasia and coronary vessel disorganization in zebrafish

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Fig. S8

Epicardial over-proliferation is prominent in v12 mutant. (A-B) Mef2 and PCNA immunostaining images show that increased proliferating cells in the v12 mutant myocardium. (C-D) Epicardial cells is labeled by Tg(tcf21:dsRed) transgenic reporter. Increased PCNA positive cells found in the epicardium region. (E-F) Increased proliferative cells in the endocardium of v12 mutant labeled by Tg(fli1a:EGFP) transgenic reporter. (G) v12mutant has more PCNA positive nuclei per 105?m² than the wild type sibling. Data are presented as mean±s.e.m.two-tailed unpaired t-test, **, P<0.01. Scale bar: 20?m.

Expression Data

Genes:	DsRed2 EGFP
Antibodies:	Ab1-mef2 Ab2-pcna Ab5-pcna
Fish:	WT vclb^gd4Gt/gd4Gt pd37Tg y1Tg vclb^gd4Gt/gd4Gt; pd37Tg vclb^gd4Gt/gd4Gt; y1Tg (all 6)
Anatomical Terms:	endocardium epicardium myocardium
Stage:	Days 14-20

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vclb^gd4Gt/gd4Gt vclb^gd4Gt/gd4Gt; pd37Tg vclb^gd4Gt/gd4Gt; y1Tg
Observed In:	endocardium cell population proliferation epicardium cell population proliferation myocardium cell population proliferation
Stage:	Days 14-20

Phenotype Detail

Acknowledgments

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