ZFIN ID: ZDB-FIG-161104-5
Saera-Vila et al., 2016 - Autophagy Regulates Cytoplasmic Remodeling During Cell Reprogramming in a Zebrafish Model of Muscle Regeneration. Autophagy   12(10):1864-1875 Full text @ Autophagy
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Conditions:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Adult

Fig. 6

Apoptosis is not activated in response to autophagy inhibition. (A) Western blot of fish treated with increasing CQ concentrations showed a dose-dependent decrease of active Caspase 3 (Casp3) in the injured muscle (see Figure S1), suggesting a role of Casp3 in muscle regeneration. Protein loading was assayed with an anti-Tubg1/tubulin antibody. U, uninjured; I, injured. (B to F) Fish were treated with or without CQ. Apoptosis was visualized by TUNEL staining; the inset shows the differential interference contrast image (pictures are representative examples of 5 fish per group). (F) High-magnification view of the box in (D) and (E)shows cytoplasmic localization of TUNEL signal in fish treated with CQ.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab10-casp3 WB WT resection: lateral rectus, chemical treatment by environment: chloroquine Adult lateral rectus regenerating tissue
WB WT resection: lateral rectus Adult lateral rectus regenerating tissue
Phenotype Details
Fish Conditions Stage Phenotype
WT resection: lateral rectus, chemical treatment by environment: chloroquine Adult lateral rectus cell death increased occurrence, abnormal
Adult lateral rectus regenerating tissue ab10-casp3 labeling amount, ameliorated
Adult regenerating tissue cell death increased occurrence, abnormal
WT resection: lateral rectus Adult lateral rectus regenerating tissue ab10-casp3 labeling increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Autophagy for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Autophagy