ZFIN Figure: Saera-Vila et al., 2016, Fig. 6

ZFIN ID: ZDB-FIG-161104-5

ZFIN ID: ZDB-FIG-161104-5

Saera-Vila et al., 2016 - Autophagy Regulates Cytoplasmic Remodeling During Cell Reprogramming in a Zebrafish Model of Muscle Regeneration. Autophagy 12(10):1864-1875 Full text @ Autophagy

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Antibody:	Ab10-casp3
Fish:	WT
Conditions:	chemical treatment by environment: chloroquine, resection: lateral rectus resection: lateral rectus
Anatomical Term:	lateral rectus regenerating tissue
Stage:	Adult

PHENOTYPE:

Fish:	WT
Conditions:	chemical treatment by environment: chloroquine, resection: lateral rectus resection: lateral rectus
Observed In:	lateral rectus cell death lateral rectus regenerating tissue regenerating tissue cell death
Stage:	Adult

Fig. 6

Apoptosis is not activated in response to autophagy inhibition. (A) Western blot of fish treated with increasing CQ concentrations showed a dose-dependent decrease of active Caspase 3 (Casp3) in the injured muscle (see Figure S1), suggesting a role of Casp3 in muscle regeneration. Protein loading was assayed with an anti-Tubg1/tubulin antibody. U, uninjured; I, injured. (B to F) Fish were treated with or without CQ. Apoptosis was visualized by TUNEL staining; the inset shows the differential interference contrast image (pictures are representative examples of 5 fish per group). (F) High-magnification view of the box in (D) and (E)shows cytoplasmic localization of TUNEL signal in fish treated with CQ.

Gene Expression Details No data available

Antibody Labeling Details

Antibody	Assay	Fish	Conditions	Stage	Anatomy
Ab10-casp3	WB	WT	resection: lateral rectus, chemical treatment by environment: chloroquine	Adult	lateral rectus regenerating tissue
	WB	WT	resection: lateral rectus	Adult	lateral rectus regenerating tissue

Phenotype Details

Fish	Conditions	Stage	Phenotype
WT	resection: lateral rectus, chemical treatment by environment: chloroquine	Adult	lateral rectus cell death increased occurrence, abnormal
		Adult	lateral rectus regenerating tissue ab10-casp3 labeling amount, ameliorated
		Adult	regenerating tissue cell death increased occurrence, abnormal
WT	resection: lateral rectus	Adult	lateral rectus regenerating tissue ab10-casp3 labeling increased amount, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Autophagy