FIGURE

Fig. 6

ID

ZDB-FIG-161104-5

Publication

Saera-Vila et al., 2016 - Autophagy Regulates Cytoplasmic Remodeling During Cell Reprogramming in a Zebrafish Model of Muscle Regeneration

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Fig. 6

Apoptosis is not activated in response to autophagy inhibition. (A) Western blot of fish treated with increasing CQ concentrations showed a dose-dependent decrease of active Caspase 3 (Casp3) in the injured muscle (see Figure S1), suggesting a role of Casp3 in muscle regeneration. Protein loading was assayed with an anti-Tubg1/tubulin antibody. U, uninjured; I, injured. (B to F) Fish were treated with or without CQ. Apoptosis was visualized by TUNEL staining; the inset shows the differential interference contrast image (pictures are representative examples of 5 fish per group). (F) High-magnification view of the box in (D) and (E)shows cytoplasmic localization of TUNEL signal in fish treated with CQ.

Expression Data

Antibody:	Ab10-casp3
Fish:	WT
Conditions:	chemical treatment by environment: chloroquine, resection: lateral rectus resection: lateral rectus
Anatomical Term:	lateral rectus regenerating tissue
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Conditions:	chemical treatment by environment: chloroquine, resection: lateral rectus resection: lateral rectus
Observed In:	lateral rectus cell death lateral rectus regenerating tissue regenerating tissue cell death
Stage:	Adult

Phenotype Detail

Acknowledgments

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