|ZFIN ID: ZDB-FIG-161104-5|
Apoptosis is not activated in response to autophagy inhibition. (A) Western blot of fish treated with increasing CQ concentrations showed a dose-dependent decrease of active Caspase 3 (Casp3) in the injured muscle (see Figure S1), suggesting a role of Casp3 in muscle regeneration. Protein loading was assayed with an anti-Tubg1/tubulin antibody. U, uninjured; I, injured. (B to F) Fish were treated with or without CQ. Apoptosis was visualized by TUNEL staining; the inset shows the differential interference contrast image (pictures are representative examples of 5 fish per group). (F) High-magnification view of the box in (D) and (E)shows cytoplasmic localization of TUNEL signal in fish treated with CQ.
|Ab10-casp3||WB||WT||resection: lateral rectus, chemical treatment by environment: chloroquine||Adult||lateral rectus regenerating tissue|
|WB||WT||resection: lateral rectus||Adult||lateral rectus regenerating tissue|
|Acknowledgments:||This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Autophagy|