ZFIN ID: ZDB-FIG-161104-6
Saera-Vila et al., 2016 - Autophagy Regulates Cytoplasmic Remodeling During Cell Reprogramming in a Zebrafish Model of Muscle Regeneration. Autophagy   12(10):1864-1875 Full text @ Autophagy
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Conditions:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Adult

Fig. 7

Autophagy is regulated by Fgf during EOM regeneration. (A, B) Craniectomy was again used to visualize LysoTracker Red-labeling in (A) wild-type (WT) fish, and (B) hsp70l:dnfgfr1a-EGFP; the inset shows an image of the GFP channel to confirm transgene expression and, consequently, Fgfr inhibition (pictures shown are representative examples of the 12 fish per group quantified). (C) Quantification of LysoTracker Red fluorescence intensity shows a clear decrease of LysoTracker Red labeling in hsp70l:dnfgfr1a-EGFP fish, indicating a lower autophagic flux. Values are averages ± SD (Student t test; ***, P < 0.001, n = 12). (D) Western blot of Sqstm1 and Lc3. The injured muscle of hsp70l:dnfgfr1a-EGFP fish showed higher levels of Sqstm1 and lower levels of Lc3-II (E), indicating again a lower autophagic flux. Protein loading was assayed with an anti-Tubg1/tubulin antibody. (D, E) Show a representative example of 3 independent experiments. U, uninjured; I, injured. * Skull base, where the pituitary is located.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP pd1Tg resection: lateral rectus, heat shock Adult lateral rectus regenerating tissue IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-map1lc3 WB pd1Tg resection: lateral rectus, heat shock Adult lateral rectus regenerating tissue
WB zf13Tg resection: lateral rectus Adult lateral rectus regenerating tissue
Ab6-sqstm1 WB pd1Tg resection: lateral rectus, heat shock Adult lateral rectus regenerating tissue
WB zf13Tg resection: lateral rectus Adult lateral rectus regenerating tissue
Phenotype Details
Fish Conditions Stage Phenotype
pd1Tg resection: lateral rectus, heat shock Adult autophagy occurrence, ameliorated
Adult lateral rectus regenerating tissue ab1-map1lc3 labeling amount, ameliorated
Adult lateral rectus regenerating tissue Ab6-sqstm1 labeling increased amount, abnormal
Adult regenerating tissue autophagy occurrence, ameliorated
zf13Tg resection: lateral rectus Adult lateral rectus regenerating tissue ab1-map1lc3 labeling increased amount, abnormal
Adult lateral rectus regenerating tissue Ab6-sqstm1 labeling increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Autophagy for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Autophagy