ZFIN ID: ZDB-FIG-161028-22
Warga et al., 2016 - Progressive loss of RacGAP1/ogre activity has sequential effects on cytokinesis and zebrafish development. Developmental Biology   418(2):307-22 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 14-19 somites to Prim-5

Fig. 2

ogre is a mutation in racgap1. (A) Genetic map of chromosome 23 showing number of recombinants and calculated distance for microsatellite markers and genes. (B) Schematic of the racgap1 cDNA showing location of termination codon in exon 10 and (C) sequence trace data. (D) Schematic of the RacGAP1 protein. MKLP1 binding region; CC, coiled-coiled domain; ECT2/FIP3 binding region; C1, atypical C1 domain; GAP, GTPase-activating domain. (E-H) Expression of racgap1 mRNA: (E) maternal expression at 1 h; and (F-H) zygotic expression at: (F) 7 h; (G) 18 h; and (H) 24 h. (I-J) Anti-sense knockdown of racgap1 mRNA by morpholino (MO) phenocopies ogre; left side: embryo, right side: DAPI-stained nuclei. (K-P) racgap1 mRNA rescues the ogre mutant phenotype. (K) racgap1 injected and (L) kaede control embryos. (M) Quantification of multinucleate cells at 10-somites. Differences between kaede and racgap1 injected mutant embryos are significantly different (p<0.01, Wilcoxon rank sum); racgap1 injected wild type and mutant embryos are also significantly different (p<0.01, Wilcoxon rank sum). (N-P) dlA expression in neuroblasts: (N) racgap1 injected and (O) kaede control embryos and (P) their average number in ogre mutants.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
racgap1 WT standard conditions 1-cell blastodisc ISH
Shield blastoderm ISH
14-19 somites brain ISH
14-19 somites nervous system ISH
Prim-5 brain ISH
Prim-5 nervous system ISH
racgap1ta52a/ta52a standard conditions 14-19 somites brain ISH
Prim-5 Not Detected brain ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-racgap1 standard conditions 14-19 somites cell binucleate, abnormal
14-19 somites head apoptotic, abnormal
14-19 somites whole organism anterior-posterior axis decreased length, abnormal
14-19 somites whole organism opacity, abnormal
racgap1ta52a/ta52a standard conditions 14-19 somites brain racgap1 expression decreased amount, abnormal
14-19 somites cell binucleate, abnormal
14-19 somites head apoptotic, abnormal
14-19 somites whole organism anterior-posterior axis decreased length, abnormal
14-19 somites whole organism opacity, abnormal
Prim-5 brain racgap1 expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 418(2), Warga, R.M., Wicklund, A., Richards, S.E., Kane, D.A., Progressive loss of RacGAP1/ogre activity has sequential effects on cytokinesis and zebrafish development, 307-22, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.