FIGURE

Fig. 6

ID

ZDB-FIG-161028-26

Publication

Warga et al., 2016 - Progressive loss of RacGAP1/ogre activity has sequential effects on cytokinesis and zebrafish development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

RacGAP1 depleted cells survive if cytokinesis is prevented. Row (1) phenotypic wild type, row (2) homozygous hrp mutants, and row (3) homozygous zom mutants. Row 1 and 2 are siblings. Column (A-C) uninjected embryos; column (D-F) morpholino (MO) knockdown embryos. (A, D) Live embryos, (B, E) DAPI stained nuclei: red arrowheads indicate sister nuclei where both nuclei are arrested in prophase; red arrow indicates two sister nuclei where one nuclei is in prophase and the other nuclei is in metaphase; yellow arrowheads indicate sister nuclei where both nuclei are in interphase; and yellow arrow indicates two sister nuclei where one nuclei is in interphase and the other nuclei is in prophase, and (C, F) anti-active Caspase 3 stained embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cdc20^ta94/ta94 fbxo5^ti245/ti245 WT + MO1-racgap1 cdc20^ta94/ta94 + MO1-racgap1 fbxo5^ti245/ti245 + MO1-racgap1
Knockdown Reagent:	MO1-racgap1
Observed In:	cell cell mitotic metaphase cell mitotic prophase cytokinesis mitotic cell cycle whole organism apoptotic process
Stage Range:	10-13 somites to 14-19 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 418(2), Warga, R.M., Wicklund, A., Richards, S.E., Kane, D.A., Progressive loss of RacGAP1/ogre activity has sequential effects on cytokinesis and zebrafish development, 307-22, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.