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ZFIN ID: ZDB-FIG-160926-35
Bhuiyan et al., 2016 - Acinetobacter baumannii phenylacetic acid metabolism influences infection outcome through a direct effect on neutrophil chemotaxis. Proceedings of the National Academy of Sciences of the United States of America   113(34):9599-604 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Day 6

Fig. S2

(A) After phagocytosis, A. baumannii is handled within acidic phagolysosomes as shown by the red fluorescence (pHrodo-dextran stain) within a neutrophilic vacuole (white arrow) at 50 min postinfection [Tg(mpx:GFP)il14 embryos with green fluorescent neutrophils were used]. In each panel, the maximum-projection z-stack image (i) is supplemented by x (ii) and y projections (iii). (B) Whole-embryo neutrophil numbers were counted using ImageJ at 48 hpf. Neutrophil numbers were decreased in csf3r MO-injected embryos and increased in irf8 MO-injected embryos (n = 15 per group pooled from three independent experiments; error bars represent mean ± SD). (C) Whole-embryo macrophage numbers were counted by using ImageJ at 48 hpf. Macrophage numbers were decreased in irf8 MO-injected embryos and were unchanged in csf3r MO-injected embryos. Asterisks represent comparison between control MO and csf3r MO or irf8 MO (n = 15 per group pooled from three independent experiments; error bars represent mean ± SD; *P ≤ 0.05 and **P < 0.01, one-way ANOVA, Kruskal-Wallis test). (D) Zebrafish infected with the Acinetobacter quorum-sensing mutant (ΔabaI) showed greater survival compared with wild-type Acinetobacter (M2 strain) and the complemented strain (pabaI) in zebrafish (P value is a comparison of M2 and ΔabaI) and (E) a murine septicemia model (n = 15 per group; P value is a comparison of M2 and ΔabaI by log-rank test).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO1-irf8 bacterial treatment by injection: Acinetobacter baumannii ATCC 17978 Long-pec - Day 6 whole organism has extra parts of type neutrophil, abnormal
Long-pec - Day 6 whole organism has fewer parts of type macrophage, abnormal
TU + MO3-csf3r bacterial treatment by injection: Acinetobacter baumannii ATCC 17978 Long-pec - Day 6 whole organism has fewer parts of type neutrophil, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA