ZFIN ID: ZDB-FIG-160915-19
Powell et al., 2016 - Wtip is required for proepicardial organ specification and cardiac left/right asymmetry in zebrafish. Molecular Medicine Reports   14(3):2665-78 Full text @ Mol. Med. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 4

Wt1a and Wtip cooperate for PE formation. Ventral view of 48 hpf whole-mount in situ hybridization of (A,C,E,G,I,K) tcf21 and (B,D,F,H,J,L) tbx18 expression in control (yellow arrowhead, A,B), high concentration of wtipMO (black arrow, C,D; 1.035 pmol/embryo), sub-threshold concentration of wtipMO (yellow arrowhead, E,F; 0.259 pmol/embryo), high concentration of wt1aMO (black arrow, G,H; 1.15 pmol/embryo), sub-threshold concentration of wt1aMO (yellow arrowhead, I,J; wt1aMO, 0.288 pmol/embryo), sub-threshold concentrations of wt1aMO (1.15 pmol/embryo)+wtipMO (0.259 pmol/embryo; black arrow, K,L) in PE at 48 hpf. With high concentrations of wt1aMO, PE marker expressions were reduced or absent (black arrow, for tcf21; G,M, and for tbx18; H,N). With wtipMO, PE marker expressions were reduced (for tcf21, M and for tbx18, N) or absent (black arrow, for tcf21; C,M, and for tbx18; D,N). Sub-threshold doses for each MO had no effect on tcf21 (yellow arrowhead, E,I,M) or tbx18 (yellow arrowhead, F,J,N) expression in PE. In the wtip and wt1a double morphants, tcf21 and tbx18 were severely reduced (black arrow, for tcf21; K,M and for tbx18; L,N) or no longer expressed (for tcf21 and for tbx18) in the cardiac region. These data indicate that Wtip is associated with Wt1a and influences PE specification. Wt1a, Wilm′s tumor 1a; Wtip, Wilm′s tumor 1 interacting protein; PR, proepicardial organ; hpf, hours post fertilization; tcf21, transcription factor 21; tbx18, Tbox 18; wtipMO, wtip morpholino oligonucleotide.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
tbx18 AB control Long-pec proepicardial cluster ISH
AB + MO2-wtip control Long-pec proepicardial cluster ISH
AB + MO2-wtip + MO4-wt1a control Long-pec Not Detected proepicardial cluster ISH
AB + MO4-wt1a control Long-pec proepicardial cluster ISH
tcf21 AB control Long-pec proepicardial cluster ISH
AB + MO2-wtip control Long-pec proepicardial cluster ISH
AB + MO2-wtip + MO4-wt1a control Long-pec Not Detected proepicardial cluster ISH
AB + MO4-wt1a control Long-pec proepicardial cluster ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-wtip control Long-pec proepicardial cluster tbx18 expression decreased amount, abnormal
Long-pec proepicardial cluster tcf21 expression decreased amount, abnormal
AB + MO2-wtip + MO4-wt1a control Long-pec proepicardial cluster tbx18 expression absent, abnormal
Long-pec proepicardial cluster tcf21 expression absent, abnormal
AB + MO4-wt1a control Long-pec proepicardial cluster tbx18 expression decreased amount, abnormal
Long-pec proepicardial cluster tcf21 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Molecular Medicine Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Mol. Med. Rep.