FIGURE

Fig. 2

ID

ZDB-FIG-160823-13

Publication

de Preux Charles et al., 2016 - Preconditioning boosts regenerative programmes in the adult zebrafish heart

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Fig. 2

Thoracotomy stimulates mitotic events in the intact heart without apoptotic turnover of the newly generated cells. (a) Representative image of a mitotic CM in an intact heart of transgenic fish cmlc2:EGFP at 7 dpt. Orthogonal projections demonstrate a colocalization between PH3 (red), GFP (green) and DAPI (blue) staining. (b,c) Quantification of PH3-positive CM and non-CM nuclei at 7 dpt. Mef2 was used as a CM nuclei marker. (d-f) Representative images of the TUNEL assay (green) at 4 dpci (days post-cryoinjury; positive control for apoptosis with the post-infarcted area surrounded with a dashed line), in uninjured hearts and at 4 dpt. (g) Quantification of TUNEL-positive nuclei in hearts at 4 dpci, in uninjured hearts and at different time points post-thoracotomy (n ≥ 4 hearts; ≥2 sections per heart; n.s., non-significant; ***p < 0.001).

Expression Data

Gene:	EGFP
Antibody:	Ab14-h3
Fish:	f1Tg
Condition:	surgical manipulation: pericardial cavity
Anatomical Term:	heart
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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