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General Information
ZIRC
ZFIN ID: ZDB-FIG-160818-24
Rochon et al., 2016 - Alk1 controls arterial endothelial cell migration in lumenized vessels. Development (Cambridge, England)   143(14):2593-602 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 4

Arterial endothelial cell displacement towards the heart requires alk1 and blood flow. (A) Representative two-dimensional confocal projections of control and alk1-morphant Tg(fli1a:GAL4FF)ubs4;Tg(UAS:kaede)rk8 embryos at 24hpf (pre- and post-photoconversion of AA1 with 405nm laser) and 48hpf. Green, native Kaede; magenta, photoconverted Kaede. Arrowheads denote segment originally photoconverted. 24hpf: merge; dorsal view, anterior down. 48hpf: 488nm laser (native Kaede; green), 516nm laser (photoconverted Kaede; magenta), and merge; frontal view, dorsal up. Trio of images at right shows a single plane of a wild-type ventricle at 48hpf, demonstrating the contribution of AA1-derived cells to the endocardium. Scale bars: 50┬Ám. (B) Schematic of 48hpf heart and cranial vasculature showing color-coded segmental boundaries used for analysis of location of photoconverted cells. (C-K) Percentage of 48hpf control (C-E), alk1-morphant (F-H) and tnnt2a-morphant (I-K) embryos exhibiting photoconverted cells in specified region of heart and cranial vessels; data are laid out proximal (left) to distal (right) with respect to the heart. Cells were photoconverted at 24hpf in proximal AA1 (C,F,I), proximal ICA (D,G,J) or at the CaDI/ICA junction (E,H,K). Gray shading denotes site of photoconversion. n (number of embryos) noted in each panel, upper right.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Kaede rk8Tg; ubs4Tg control Prim-5 aortic arch 1 IFL
Prim-5 caudal division of the internal carotid artery IFL
Prim-5 cranial vasculature IFL
Prim-5 internal carotid artery IFL
Long-pec aortic arch 1 IFL
Long-pec bulbus arteriosus IFL
Long-pec cardiac ventricle IFL
Long-pec caudal division of the internal carotid artery IFL
Long-pec cranial vasculature IFL
Long-pec endocardium IFL
Long-pec internal carotid artery IFL
rk8Tg; ubs4Tg + MO3-acvrl1 control Prim-5 aortic arch 1 IFL
Prim-5 caudal division of the internal carotid artery IFL
Prim-5 cranial vasculature IFL
Prim-5 internal carotid artery IFL
Long-pec aortic arch 1 IFL
Long-pec bulbus arteriosus IFL
Long-pec cardiac ventricle IFL
Long-pec caudal division of the internal carotid artery IFL
Long-pec cranial vasculature IFL
Long-pec endocardium IFL
Long-pec internal carotid artery IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rk8Tg; ubs4Tg + MO1-tnnt2a control Prim-5 - Long-pec aortic arch 1 blood vessel endothelial cell migration process quality, abnormal
Prim-5 - Long-pec aortic arch 1 cell migration involved in heart development decreased occurrence, abnormal
Prim-5 - Long-pec blood circulation decreased occurrence, abnormal
Prim-5 - Long-pec caudal division of the internal carotid artery blood vessel endothelial cell migration process quality, abnormal
Prim-5 - Long-pec cell migration involved in heart development decreased occurrence, abnormal
Prim-5 - Long-pec internal carotid artery blood vessel endothelial cell migration process quality, abnormal
rk8Tg; ubs4Tg + MO3-acvrl1 control Prim-5 - Long-pec aortic arch 1 blood vessel endothelial cell migration process quality, abnormal
Prim-5 - Long-pec aortic arch 1 cell migration involved in heart development decreased occurrence, abnormal
Prim-5 - Long-pec blood circulation decreased occurrence, abnormal
Prim-5 - Long-pec caudal division of the internal carotid artery blood vessel endothelial cell migration process quality, abnormal
Prim-5 - Long-pec cell migration involved in heart development decreased occurrence, abnormal
Prim-5 - Long-pec internal carotid artery blood vessel endothelial cell migration process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development