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ZFIN ID: ZDB-FIG-160816-9
Nord et al., 2016 - Pax7 is required for establishment of the xanthophore lineage in zebrafish embryos. Molecular biology of the cell   27(11):1853-62 Full text @ Mol. Biol. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 4 The pax7a/pax7b double-mutant embryos have a reduced xanthoblast pool and completely lack differentiated xanthophores. Whole-mount in situ hybridization on wt siblings and pax7a/pax7b double-mutant zebrafish embryo at 24 hpf showing the expression of (A) gch2 (n = 13 for siblings and n = 7 for pax7a/pax7b double mutants), (B) csf1r (n = 5 and 3), and (C) xdh (n = 3 and 5). (D) Pteridine autofluorescence in wt and pax7a/pax7b double-mutant zebrafish embryo at 48 hpf. Box indicates area of enlargement visualized in insets. Black arrowheads indicate head region where changes in expression can be detected; white arrowheads indicate a subset of autofluorescing xanthophores. Scale bar, 200 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
csf1ra WT standard conditions Prim-5 neural crest cell ISH
pax7bumu4/umu4 ; pax7aumu3/umu3 standard conditions Prim-5 neural crest cell ISH
gch2 WT standard conditions Prim-5 xanthoblast ISH
pax7bumu4/umu4 ; pax7aumu3/umu3 standard conditions Prim-5 xanthoblast ISH
xdh WT standard conditions Prim-5 neural crest cell ISH
pax7bumu4/umu4 ; pax7aumu3/umu3 standard conditions Prim-5 neural crest cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
pax7bumu4/umu4 ; pax7aumu3/umu3 standard conditions Prim-5 whole organism has fewer parts of type xanthoblast, abnormal
Prim-5 xanthophore differentiation decreased process quality, abnormal
Long-pec whole organism lacks all parts of type xanthophore, abnormal
Long-pec xanthophore differentiation decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Molecular biology of the cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Mol. Biol. Cell