The number of melanophores is decreased in pax7a/pax7b double-mutant fish during metamorphosis. (A) Pigment phenotype of wt, pax7a-/-, pax7b-/-, and pax7a/pax7b double-mutant juvenile zebrafish at 30 dpf. Box indicates area of melanophore quantification presented in C. (B) Enlargement of area counted in C. (C) Number of melanophores in wt (n = 6), pax7a-/- (n = 8), pax7b-/- (n = 6), and pax7a/pax7b double-mutant (n = 10) juvenile zebrafish at 30 dpf. The pax7a/pax7b double-mutant zebrafish differs from all other groups with p < 0.001. Comparisons between groups by one-way ANOVA or Kruskal–Wallis ANOVA on ranks with Student’s–Newman–Keuls or Dunn’s post hoc contrast test for parametric and nonparametric data. Scale bar, 2 mm (A), 200 μm (B).
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