FIGURE

Fig. 4

ID

ZDB-FIG-160708-5

Publication

He et al., 2016 - Synergy between loss of NF1 and overexpression of MYCN in neuroblastoma is mediated by the GAP-related domain

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Fig. 4

Loss of nf1 promotes neuroblastoma tumor progression in MYCN transgenic fish.

(A) Neuroblastoma development in representative fish of the nf1a+/+;nf1b+/+;MYCN;GFP, nf1a+/-;nf1b+/+;MYCN;GFP and nf1a-/-;nf1b+/+;MYCN;GFP genotypes over 3 to 6 weeks of age.Each fish was imaged weekly for 3 continuous weeks from 3 weeks of age. (B) Quantification of GFP+ sympathoadrenal cells in the IRG of fish with nf1a+/+;nf1b+/+;MYCN;GFP (n = 10), nf1a+/-;nf1b+/+;MYCN;GFP (n = 21) and nf1a-/-;nf1b+/+;MYCN;GFP (n = 15) genotypes, demonstrating tumor progression over 3 weeks. Tumors were scored when the GFP+ signals in the IRG exceeded the threshold defined by the dotted line. (C) IRG development in nf1a mutant zebrafish lacking overexpression of MYCN. Quantification of GFP+ sympathoadrenal cells in the IRG of fish with nf1a+/+;nf1b+/+;GFP (n = 10), nf1a+/-;nf1b+/+;GFP (n = 10) and nf1a-/-;nf1b+/+;GFP (n = 10) genotypes. The same tumor threshold line shown in panel B is included for comparison.

Expression Data

Gene:	EGFP
Fish:	zdf16Tg nf1a^p301/+; zdf16Tg nf1a^p301/p301; zdf16Tg
Anatomical Term:	interrenal gland neuroblastoma
Stage Range:	Days 21-29 to Days 30-44

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zdf16Tg nf1a^p301/+; zdf16Tg nf1a^p301/p301; zdf16Tg
Observed In:	interrenal gland neuroblastoma
Stage Range:	Days 21-29 to Days 30-44

Phenotype Detail

Acknowledgments

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