FIGURE

Fig. 2

ID

ZDB-FIG-160708-2

Publication

He et al., 2016 - Synergy between loss of NF1 and overexpression of MYCN in neuroblastoma is mediated by the GAP-related domain

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Fig. 2

Loss of nf1 accelerates disease onset and increases the penetrance of MYCN-induced neuroblastoma.

(A) Representative fish of nf1a+/+;nf1b+/+;GFP, nf1a+/+;nf1b+/+;MYCN;GFP, nf1a-/-;nf1b+/+;GFP and nf1a-/-;nf1b+/+;MYCN;GFP genotypes at 5 weeks of age.The interrenal glands (IRGs) are highlighted with dashed circles. (B) H&E-stained sagittal sections of a 10-week old nf1a+/+;nf1b+/+;GFP fish (left) and a 10-week old nf1a-/-;nf1b+/-;GFP;MYCN fish (middle) and 63-fold magnified tumor cells (right), which are magnified from the area in the small white box in the middle panel. (C) Cumulative frequency of neuroblastoma in MYCN transgenic zebrafish representing all eight nf1 genotypes generated by the breeding of the nf1a+/-;nf1b+/-;MYCN line with the nf1a+/-;nf1b+/- zebrafish line (*p<0.0001 nf1a-/-;nf1b+/+ vs. nf1a+/+;nf1b+/+).

Expression Data

Gene:	EGFP
Fish:	zdf15Tg nf1a^p301/p301; zdf15Tg zdf16Tg nf1a^p301/p301; zdf16Tg
Anatomical Term:	superior cervical ganglion
Stage:	Days 30-44

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zdf16Tg nf1a^p301/+; zdf16Tg nf1a^p301/p301; zdf16Tg nf1b^p304/+; zdf16Tg nf1b^p304/p304; zdf16Tg nf1a^p301/+; nf1b^p304/+; zdf16Tg nf1a^p301/+; nf1b^p304/p304; zdf16Tg nf1a^p301/p301; nf1b^p304/+; zdf16Tg
Observed In:	interrenal gland neuroblastoma
Stage Range:	Days 21-29 to Adult

Phenotype Detail

Acknowledgments

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