Fig. 5

duox is highly expressed around tectal ventricle at 48 hpf . A-D: Lateral views of whole-mount ISH embryos probed with antisense (A-C) and sense control (D) riboprobe against zebrafish duox mRNA. Lines represent the position of sections shown in E-X. E-H: 10-µm-thick transverse sections through the forebrain (line labeled “f” in A) of 24, 36, and 48 hpf embryos incubated with antisense probes (E-G, respectively) and 24 hpf embryo probed with a sense control (H). I-L: Transverse sections through the eye (line labeled “e” in A). M-P: Corresponding midbrain sections (line labeled “m” in A). High duox expression was detected around the tectal ventricle (arrows in O). Q-T: Corresponding hindbrain sections (line labeled “h” in A). U-X: Corresponding spinal sections (line labeled “s” in A). Asterisk and arrows represent regions and small areas of increased duox expression in the spinal cord and midbrain, respectively. Abbreviations: 3V, third ventricle; DC, diencephalon; E, eye; MC, mesencephalon; MyC, myelencephalon; NC, notochord; ON, optic nerve; OV, otic vesicle; SC, spinal cord; SM, somites; TC, telencephalon; Tec, tectum; TecV, tectal ventricle. Scale bar = 0.5 mm in A-D; 100 µm in E-X.