Community Action Needed: Please respond to the NIH RFI
ZFIN ID: ZDB-FIG-160524-14
Eno et al., 2016 - aura/mid1ip1L regulates the cytoskeleton at the zebrafish egg-to-embryo transition. Development (Cambridge, England)   143(9):1585-99 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: 1-cell
PHENOTYPE:
Fish:
Observed In:
Stage: 1-cell

Fig. S5

Membrane dynamics of wild-type and aura mutant embryos. (A-D) Side view of wild-type (A,B) and aura mutant (C,D) embryos at 2 mpf and 10 mpf, labeled to detect F-actin and CGs, showing that mutants exhibits a similar localization of CGs at the cortex during egg activation (A,C) and retains a fraction of CGs (B,D). (E-F) In wild-type at 45 mpf, β-catenin localizes to the furrow but Vamp2 does not yet localize to this structure (4/4 embryos) (E, E′) In aura mutants, Vamp2 appears localize to ectopic CGs (8/9 embryos) (F,F′). Vamp2 also localizes to small cortical particles in both wild-type and mutant embryos (E′, F′). (G-L) Localization of Clathrin (G, G′), Dynamin2 (H,H′), Rab11 (I,I′), Vamp2 (J,J′), Vamp4 (K,K′), and ß-catenin (L,L′) to a subset of CGs in wild-type at 2 mpf (G-H) or 0 mpf (I-L). (M, M′) Dynamin2 surrounding CGs in aura mutant embryos as seen by a face-on view (M) and a reconstructed orthogonal view (M′). Scale bars: A-D (bar in A): 10 µm; E-H (bar in E): 100 µm; E′-H′(bar in E′): 10 µm; I-M′ (bar in I,I′,M): 10 µm.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-dnm2 IHC AB standard conditions 1-cell blastomere cortical granule
IHC mid1ip1lt9792/t9792 standard conditions 1-cell blastomere
Ab1-rab11ba IHC AB standard conditions 1-cell blastomere cortical granule
Ab1-vamp2 IHC AB standard conditions 1-cell blastomere
IHC 1-cell blastomere cortical granule
IHC mid1ip1lt9792/t9792 standard conditions 1-cell blastomere cortical granule
Ab2-cltc IHC AB standard conditions 1-cell blastomere cleavage furrow
IHC 1-cell blastomere cortical granule
IHC mid1ip1lt9792/t9792 standard conditions 1-cell blastomere cleavage furrow
Ab2-ctnnb1 IHC AB standard conditions 1-cell blastomere cortical granule
Phenotype Details
Fish Conditions Stage Phenotype
mid1ip1lt9792/t9792 standard conditions 1-cell blastomere cleavage furrow Ab2-cltc labeling decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development