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ZFIN ID: ZDB-FIG-160524-12
Eno et al., 2016 - aura/mid1ip1L regulates the cytoskeleton at the zebrafish egg-to-embryo transition. Development (Cambridge, England)   143(9):1585-99 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Sphere to 50%-epiboly
PHENOTYPE:
Fish:
Observed In:
Stage: 64-cell

Fig. S3

Overview of landmarks of oogenesis and early cleavage in aura mutants. (A-D) Landmarks during oogenesis appear normal in aura oocytes. (A,B) Accumulation of F-actin appears unaffected normal in stage I aura mutant oocytes (wt= 5/5, aura= 4/4). Accumulation and localization of mitochondria and ER, marking the Balbiani body (Gupta et al. 2010), appears normal in stage I aura mutant oocytes (wt= 23/23, aura= 20/20). (E-H) Loss of blastomere cellular adhesion in aura mutant embryos. Live imaging of F-actin in embryos using the Life Act transgene in wild-type (3/3 E,G) and aura mutant line (3/3 F,H). The cellular pattern is relatively normal up to the 8-cell stage, as expected since cell adhesion is a property of mature furrows that become fully formed for the first cell cycle at this stage. By late 16-cell stage, aura embryos begin to display rounded and loose cells, which can be of varying sizes, consistent with defects in late cytokinesis involving reduced adhesive membrane deposition and membrane regression. The normal cleavage pattern up to the 8-cell stage indicates that the spindle orientation that results in the typically invariant cleavage pattern is normal. Images were taken in one minute intervals from 60 mpf to 85 mpf (E,F) and two minute intervals from 90 mpf to 120 mpf (G,H). (I-L) In aura mutants, DNA masses undergo epiboly-like vegetal movement in the absence of cellular layers. The DNA masses are uneven, likely due to the lack of cell membranes in these embryos and consequent mis-segregation of chromosomes by neighboring asters. Top panels (I,J, animal view) are pre-epiboly stages (4 hpf), bottom panels (K,L, side view) are 50% epiboly stages (5.5 hpf). DNA masses in aura mutants (J,L) appear at vegetal levels consistent with an epibolic-like movement, similar to migration in wild-type (I,K) except with reduced cell membrane formation. β-catenin (green, highlights membrane, which is reduced in mutants), DNA (blue). Scale bars: A-D: 25 µm; I-L: 100 µm

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-ctnnb1 IHC AB standard conditions Sphere blastomere
IHC 50%-epiboly whole organism
IHC mid1ip1lt9792/t9792 standard conditions Sphere blastomere
IHC 50%-epiboly whole organism
Phenotype Details
Fish Conditions Stage Phenotype
e114Tg ; mid1ip1lt9792/t9792 standard conditions 64-cell blastomere cleavage furrow morphology, abnormal
16-cell blastomere cleavage furrow morphology, abnormal
32-cell blastomere cleavage furrow morphology, abnormal
64-cell whole organism morphology, abnormal
16-cell whole organism morphology, abnormal
32-cell whole organism morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development