FIGURE

Fig. 5

ID

ZDB-FIG-160519-6

Publication

Zhang et al., 2016 - A Founder Mutation in VPS11 Causes an Autosomal Recessive Leukoencephalopathy Linked to Autophagic Defects

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Fig. 5

Significant cell death is observed in the CNS of zebrafish vps11(plt) mutants.TUNEL assay was performed on cryosectioned CNS tissue from vps11(plt) mutants and wild-type siblings at 3, 5, and 7 days post-fertilization (dpf). (A-C) Minimal apoptotic TUNEL+ cells were observed in the hindbrain of vps11(plt) mutants at 3, 5, and 7 dpf, respectively. (D-E) Tissue section of the midbrain of a wild-type control animal at 7 dpf showing minimal cell death (panel E, arrow). (F-G) Tissue section of the midbrain of a vps11(plt) mutant at 7 dpf showing extensive cell death. (H) Quantification of the average number of TUNEL+ cells observed in the hindbrain in vps11(plt) mutants and wild-type siblings at 3, 5, and 7 dpf. No significant differences were observed. (I) Quantification of the average number of TUNEL+ cells observed in the midbrain in vps11(plt) mutants and wild-type siblings at 3, 5, and 7 dpf. Asterisk indicates significantly different from control.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vps11^wsu1/wsu1 (AB)
Observed In:	midbrain cell death
Stage Range:	Protruding-mouth to Days 7-13

Phenotype Detail

Acknowledgments

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