FIGURE

Fig. 6

ID

ZDB-FIG-160429-19

Publication

Lipscomb et al., 2016 - Dasatinib as a treatment for Duchenne muscular dystrophy

Other Figures

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Fig. 6

The effect of MG132 treatment on levels of phosphorylated and non-phosphorylated β-dystroglycan in sapje zebrafish larvae. Lysates were made from sapje/ embryos treated from 24 hpf until 96 hpf with 5 μM MG132 or DMSO-only. (A) Western blots probed with antibodies for β-dystroglycan (β-DG) and α-tubulin (α-tub). (B) The density of the blot probed with β-DG was quantified relative to α-tubulin levels in each sample, and normalized to the average control signal. There was a significant increase in the level of β-dystroglycan in larvae treated with MG132, compared with controls (unpaired t-test: t = 4.048, df = 10, P = 0.0023). (C) Western blots probed with antibodies for phosphorylated β-dystroglycan (p-β-DG) and α-tubulin. (D) The density of the blot probed with p-β-DG was quantified relative to α-tubulin levels in each sample, and normalized to the average control signal. There was a significant increase in the levels of β-DG in larvae between different treatment groups (unpaired t-test: t = 11.47, df = 10, P < 0.0001). Graphs represent the mean of six samples from three independent experiments, error bars are SEM.

Expression Data

Gene:	dag1
Antibodies:	Ab4-dag1 Ab5-dag1
Fish:	dmd^{ta222a/ta222a}
Condition:	chemical treatment: N-benzyloxycarbonyl-L-leucyl-L-leucyl-L-leucinal
Anatomical Term:	whole organism
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dmd^{ta222a/ta222a}
Condition:	chemical treatment: N-benzyloxycarbonyl-L-leucyl-L-leucyl-L-leucinal
Observed In:	whole organism
Stage:	Day 4

Phenotype Detail

Acknowledgments

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