ZFIN ID: ZDB-FIG-160425-1
Nishioka et al., 2016 - ATX-LPA1 axis contributes to proliferation of chondrocytes by regulating fibronectin assembly leading to proper cartilage formation. Scientific Reports   6:23433 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: Day 4 to Day 5

Fig. 1

Loss of ATX-LPA1 signaling resulted in dyschondroplasia in zebrafish.

(a,b) Loss of ATX- LPA1 signaling leads to deformation of the cephalic region in zebrafish embryos. Cephalic regions of wt and lpa1 mutant zebrafish embryos (a) and cephalic regions of zebrafish embryos treated with LPA1 antagonist, LPA1 or ATX morpholinos (b) at 96 hpf (side view). Cartilage tissues visualized by alcian blue and alizarin red staining are also shown (ventral view). Scale bar: 100 µm. (c) Both lpa1 and atx are co-expressed with sox9, a marker of chondrocytes, in zebrafish embryo both at 72 and 96 hpf as judged by in situ hybridization. Scale bar: 50 µm. (d,e) Loss of ATX-LPA1 signaling in zebrafish embryos leads to mislocalization of chondrocytes in cartilage tissues. (d) EGFP is expressed specifically in chondrocytes in col2:egfp transgenic zebrafish at 120 hpf. Scale bar: 100 µm. (e) In zebrafish embryos treated with LPA1 or ATX morpholinos, chondrocytes are unevenly distributed in Meckel’s and ceratohyal cartilages at 120 hpf. Scale bar: 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zf678Tg standard conditions Day 5 chondrocyte IFL
enpp2 AB standard conditions Protruding-mouth chondrocyte ISH
Day 4 chondrocyte ISH
lpar1 AB standard conditions Protruding-mouth chondrocyte ISH
Day 4 chondrocyte ISH
sox9a AB standard conditions Protruding-mouth chondrocyte ISH
Day 4 chondrocyte ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB chemical treatment: KI16425 Day 4 cartilage development process quality, abnormal
Day 4 ceratohyal cartilage deformed, abnormal
Day 4 cranium malformed, abnormal
Day 4 head circular, abnormal
Day 4 Meckel's cartilage deformed, abnormal
AB + MO1-enpp2 standard conditions Day 4 cartilage development process quality, abnormal
Day 4 ceratohyal cartilage deformed, abnormal
Day 4 cranium malformed, abnormal
Day 4 head circular, abnormal
Day 4 Meckel's cartilage deformed, abnormal
AB + MO1-lpar1 standard conditions Day 4 cartilage development process quality, abnormal
Day 4 ceratohyal cartilage deformed, abnormal
Day 4 cranium malformed, abnormal
Day 4 head circular, abnormal
Day 4 Meckel's cartilage deformed, abnormal
AB + MO2-enpp2 standard conditions Day 4 cartilage development process quality, abnormal
Day 4 ceratohyal cartilage deformed, abnormal
Day 4 cranium malformed, abnormal
Day 4 head circular, abnormal
Day 4 Meckel's cartilage deformed, abnormal
lpar1vu374/vu374 standard conditions Day 4 cartilage development process quality, abnormal
Day 4 ceratohyal cartilage decreased length, abnormal
Day 4 ceratohyal cartilage deformed, abnormal
Day 4 cranium malformed, abnormal
Day 4 head circular, abnormal
Day 4 Meckel's cartilage decreased length, abnormal
Day 4 Meckel's cartilage deformed, abnormal
zf678Tg + MO1-enpp2 standard conditions Day 5 ceratohyal cartilage chondrocyte disorganized, abnormal
Day 5 ceratohyal cartilage chondrocyte increased variability of size, abnormal
Day 5 Meckel's cartilage chondrocyte disorganized, abnormal
Day 5 Meckel's cartilage chondrocyte increased variability of size, abnormal
zf678Tg + MO1-lpar1 standard conditions Day 5 ceratohyal cartilage chondrocyte disorganized, abnormal
Day 5 ceratohyal cartilage chondrocyte increased variability of size, abnormal
Day 5 Meckel's cartilage chondrocyte disorganized, abnormal
Day 5 Meckel's cartilage chondrocyte increased variability of size, abnormal
zf678Tg + MO2-enpp2 standard conditions Day 5 ceratohyal cartilage chondrocyte disorganized, abnormal
Day 5 ceratohyal cartilage chondrocyte increased variability of size, abnormal
Day 5 Meckel's cartilage chondrocyte disorganized, abnormal
Day 5 Meckel's cartilage chondrocyte increased variability of size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.