FIGURE

Fig. 1

ID

ZDB-FIG-160324-20

Publication

Hoffman et al., 2016 - Estrogens Suppress a Behavioral Phenotype in Zebrafish Mutants of the Autism Risk Gene, CNTNAP2

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Fig. 1

cntnap2ab Mutants Display GABAergic Deficits
(A) Cntnap2a and Cntnap2b proteins showing functional domains. DISC, discoidin; EGF, epidermal growth factor; 4.1, 4.1 binding domain; LamG, laminin G; TM, transmembrane.
(B) Western blot analysis of Cntnap2a and Cntnap2b in cntnap2a^Δ25/Δ25cntnap2b^Δ7/Δ7 and cntnap2a^Δ121/Δ121cntnap2b^31i/31i fish.
(C-F and C′-F′) Reporter gene expression in Tg(dlx6a-1.4kbdlx5a/dlx6a:GFP) and Tg(vglut:DsRed) in wild-type (C-F) and cntnap2a^Δ121/Δ121cntnap2b^31i/31i (cntnap2ab) (C′-F′) larvae at 4 dpf. Note the deficit in dlx5a/6a:GFP+ cells in the telencephalon (arrowheads). (C and D, C′ and D′) Lateral views. (E and F, E′ and F′) Ventral views. hyp, hypothalamus; OT, optic tectum; tel, telencephalon.
(G) Total number of dlx5a/6a:GFP+ cells in the forebrain of wild-type (n = 13) and cntnap2ab mutants (n = 14). *p = 3.08 × 10⁷ (one-way ANOVA).
(H) Average activity in response to PTZ (10 mM) in the progeny of incrossed cntnap2a^Δ25/+cntnap2b^Δ7/+ fish at 4 dpf (n = 268; cntnap2ab + PTZ, n = 6; wild-type + PTZ, n = 11; cntnap2ab + water, n = 11; wild-type + water, n = 6; p = 0.0013, two-way ANOVA, genotype × dose interaction).

Expression Data

Genes:	DsRed GFP
Antibodies:	Ab1-cntnap2 Ab1-tuba
Fish:	WT cntnap2a^{ya1847/ya1847} cntnap2a^{ya2188/ya2188} cntnap2b^{ya1955/ya1955} cntnap2b^{ya2043/ya2043} nns14Tg; ot1Tg cntnap2a^ya1847/+; cntnap2b^ya1955/+ cntnap2a^{ya1847/ya1847}; cntnap2b^{ya1955/ya1955} cntnap2a^{ya1847/ya1847}; cntnap2b^{ya1955/ya1955}; nns14Tg; ot1Tg cntnap2a^ya2188/+; cntnap2b^ya2043/+ cntnap2a^{ya2188/ya2188}; cntnap2b^{ya2043/ya2043}
Anatomical Terms:	GABAergic neuron glutamatergic neuron hypothalamus optic tectum telencephalon white matter whole organism
Stage Range:	Protruding-mouth to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cntnap2a^{ya1847/ya1847}; cntnap2b^{ya1955/ya1955} cntnap2a^{ya1847/ya1847}; cntnap2b^{ya1955/ya1955}; nns14Tg; ot1Tg cntnap2a^{ya2188/ya2188}; cntnap2b^{ya2043/ya2043}
Condition:	chemical treatment: pentetrazol
Observed In:	brain cerebellum forebrain GABAergic neuron glutamatergic neuron locomotion whole organism
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

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