Fig. 1

Fig. 1

cntnap2ab Mutants Display GABAergic Deficits
(A) Cntnap2a and Cntnap2b proteins showing functional domains. DISC, discoidin; EGF, epidermal growth factor; 4.1, 4.1 binding domain; LamG, laminin G; TM, transmembrane.
(B) Western blot analysis of Cntnap2a and Cntnap2b in cntnap2a^Δ25/Δ25cntnap2b^Δ7/Δ7 and cntnap2a^Δ121/Δ121cntnap2b^31i/31i fish.
(C-F and C′-F′) Reporter gene expression in Tg(dlx6a-1.4kbdlx5a/dlx6a:GFP) and Tg(vglut:DsRed) in wild-type (C-F) and cntnap2a^Δ121/Δ121cntnap2b^31i/31i (cntnap2ab) (C′-F′) larvae at 4 dpf. Note the deficit in dlx5a/6a:GFP+ cells in the telencephalon (arrowheads). (C and D, C′ and D′) Lateral views. (E and F, E′ and F′) Ventral views. hyp, hypothalamus; OT, optic tectum; tel, telencephalon.
(G) Total number of dlx5a/6a:GFP+ cells in the forebrain of wild-type (n = 13) and cntnap2ab mutants (n = 14). *p = 3.08 × 10⁷ (one-way ANOVA).
(H) Average activity in response to PTZ (10 mM) in the progeny of incrossed cntnap2a^Δ25/+cntnap2b^Δ7/+ fish at 4 dpf (n = 268; cntnap2ab + PTZ, n = 6; wild-type + PTZ, n = 11; cntnap2ab + water, n = 11; wild-type + water, n = 6; p = 0.0013, two-way ANOVA, genotype × dose interaction).