ZFIN ID: ZDB-FIG-160315-10
Samuel et al., 2016 - Six3 regulates optic nerve development via multiple mechanisms. Scientific Reports   6:20267 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-15

Fig. 5

Abnormal midline signalling in Six3-deficient embryos.

(A-H) Expression of signalling molecules in anterior diencephalic midline of normal (A,C,E,G) and Six3-deficient (B,D,F,H) 32 hpf embryos. (A-F) frontal views, (A′-F′) the same embryos as in (A-F) respectively, lateral views. (A,A′,B,B′) slit1a expression. Asterisk in (B) marks an apparently single commissure. (C,C′,D,D′) slit2 expression. Asterisk in (D) marks what appears as a single commissure. (E,E′,F,F′) slit3 expression. Asterisk in F marks the reduced POA. Brackets in all lateral views mark the POA flanked by AC and POC. (G,H) sema3d expression. Arrows point at the POA. AC, anterior commissure; POA, preoptic area; POC, post-optic commissure; t, telencephalon. (G,H) are dorsal views, anterior down. Anterior is to the left in lateral views. Scale bars are 50 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
sema3d AB/TL control Prim-15 diencephalon antero-medial region ISH
Prim-15 preoptic area ISH
six3avu129/vu129 ; six3bvu87/vu87(AB/TL) control Prim-15 diencephalon antero-medial region ISH
Prim-15 preoptic area ISH
slit1a AB/TL control Prim-15 diencephalon antero-medial region ISH
Prim-15 preoptic area ISH
six3avu129/vu129 ; six3bvu87/vu87(AB/TL) control Prim-15 diencephalon antero-medial region ISH
Prim-15 preoptic area ISH
slit2 AB/TL control Prim-15 diencephalon antero-medial region ISH
Prim-15 preoptic area ISH
six3avu129/vu129 ; six3bvu87/vu87(AB/TL) control Prim-15 diencephalon antero-medial region ISH
Prim-15 preoptic area ISH
slit3 AB/TL control Prim-15 diencephalon antero-medial region ISH
Prim-15 preoptic area ISH
six3avu129/vu129 ; six3bvu87/vu87(AB/TL) control Prim-15 diencephalon antero-medial region ISH
Prim-15 preoptic area ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
six3avu129/vu129 ; six3bvu87/vu87(AB/TL) control Prim-15 diencephalon antero-medial region sema3d expression decreased amount, abnormal
Prim-15 diencephalon antero-medial region slit1a expression decreased amount, abnormal
Prim-15 diencephalon antero-medial region slit2 expression decreased amount, abnormal
Prim-15 diencephalon antero-medial region slit3 expression decreased amount, abnormal
Prim-15 postoptic commissure adjacent to anterior commissure, abnormal
Prim-15 postoptic commissure fused with anterior commissure, abnormal
Prim-15 preoptic area sema3d expression decreased amount, abnormal
Prim-15 preoptic area slit1a expression decreased amount, abnormal
Prim-15 preoptic area slit2 expression decreased amount, abnormal
Prim-15 preoptic area slit3 expression decreased amount, abnormal
Prim-15 preoptic area decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.