FIGURE

Fig. 4

ID

ZDB-FIG-160210-17

Publication

Kim et al., 2015 - Cartilage development requires the function of Estrogen-related receptor alpha that directly regulates sox9 expression in zebrafish

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Fig. 4

ESRRa regulates expression of genes essential for cartilage development.

(A–N) Embryos at 1-cell stage were injected with either MOctrl or MOesrra, raised and analysed at the indicated stages for expression of sox9a, sox9b,sox5, sox6 and col2a1 by in situ hybridization. At 2- and 3 dpf, expression of sox9 (A–H) and col2a1 (M and N) is specifically decreased in the branchial arches in MOesrra-injected embryos, although it remains comparable in other expression domains as compared to that in control. Expression of sox5 and sox6 at 2 dpf is also substantially decreased in MOesrra-injected embryos as compared to that in control (I–L). White arrows or bars indicate branchial arches and insets shown are magnified views of the branchial regions.

Expression Data

Genes:	col2a1a sox5 sox6 sox9a sox9b
Fish:	WT WT + MO3-esrra + MO4-esrra
Knockdown Reagents:	MO3-esrra MO4-esrra
Anatomical Term:	pharyngeal arch 3-7
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-esrra + MO4-esrra
Knockdown Reagents:	MO3-esrra MO4-esrra
Observed In:	pharyngeal arch 3-7 pharyngeal arch 3-7 cartilage development
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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