FIGURE

Fig. 5

ID

ZDB-FIG-160208-56

Publication

Matrone et al., 2015 - CDK9 and its repressor LARP7 modulate cardiomyocyte proliferation and response to injury in the zebrafish heart

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Fig. 5

Effects of Cdk9 modulation on ventricle cardiomyocyte proliferation during early development of the zebrafish heart. Effects of flavopiridol (A, white bars, controls; black bars, flavopiridol 3µmol/l) on the total number of cardiomyocytes in the isolated embryonic zebrafish ventricle. Effects of Cdk9 morpholino (B, Cdk9-Mo-SB, Cdk-Mo) and Larp7 morpholino (C, Larp7-Mo-SB, Larp7-Mo) on total cardiomyocyte number in the embryonic ventricle (white bars, controls, appropriate mismatch morpholino; dark bars, splice-blocking morpholino. Effects of flavopiridol on the number of proliferating cardiomyocytes (D, white bars, controls; black bars, flavopiridol 3µmol/l). Representative confocal images (E) of isolated embryonic myl7:GFP hearts stained with DAPI (blue) and for phospho-Histone-H3 (PHH3, red nuclei, marking proliferating cardiomyocytes). Data are mean±s.e.m., n=3 experiments, n=5 hearts per group, *P<0.05, **P<0.01 ***P<0.001, two-way ANOVA test. Means±s.e.m. are shown in A, B, C and D.

Expression Data

Gene:	GFP
Fish:	f1Tg
Condition:	chemical treatment: alvocidib
Anatomical Terms:	cardiac muscle cell heart
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	f1Tg f1Tg + MO2-larp7 f1Tg + MO3-cdk9
Condition:	chemical treatment: alvocidib
Knockdown Reagents:	MO2-larp7 MO3-cdk9
Observed In:	cardiac ventricle cardiac muscle cell cardiac ventricle cardiac muscle cell proliferation
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.