FIGURE

Fig. 4

ID

ZDB-FIG-160208-55

Publication

Matrone et al., 2015 - CDK9 and its repressor LARP7 modulate cardiomyocyte proliferation and response to injury in the zebrafish heart

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Fig. 4

Effects of Larp7 knockdown on cardiac structure and function. (A) Larp7-targeting morpholinos [either splice blocking (SB) or translation blocking (TB)] resulted in normal heart development, here shown in vivo (left hand panel) and following H&E staining (right hand panel). Ventricular diastolic area (B) and ejection fraction (C) in Larp7-knockdown embryos were similar to controls (Larp7 mismatch morpholino). A mild dilatation of the atrium (D) was observed in Larp7-knockdown (TB and SB Larp7-Mo) embryos. Larp7 mRNA (E) and protein (F) were significantly reduced at 48 and 96hpf. Larp7-Mo-TB (larp7 Mo) injection caused increased phosphorylation of Ser2 in the RNA polymerase II C-terminal domain (P-Ser2 CTD), suggesting an upregulation of Cdk9 activity. n=3 experiments, *P<0.05, **P<0.01, ***P<0.001, two-way ANOVA followed by Bonferroni′s post-hoc test. Means±s.e.m. are shown in B, C, D and E.

Expression Data

Gene:	larp7
Antibodies:	Ab1-larp7 Ab3-polr2a
Fish:	f1Tg + MO2-larp7 f1Tg + MO3-larp7
Knockdown Reagents:	MO2-larp7 MO3-larp7
Anatomical Term:	whole organism
Stage Range:	Long-pec to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	f1Tg + MO2-larp7 f1Tg + MO3-larp7
Knockdown Reagents:	MO2-larp7 MO3-larp7
Observed In:	atrium whole organism
Stage Range:	Long-pec to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.