ZFIN Image: Matrone et al., 2015, Fig. 5

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Figure Caption

Fig. 5

Effects of Cdk9 modulation on ventricle cardiomyocyte proliferation during early development of the zebrafish heart. Effects of flavopiridol (A, white bars, controls; black bars, flavopiridol 3µmol/l) on the total number of cardiomyocytes in the isolated embryonic zebrafish ventricle. Effects of Cdk9 morpholino (B, Cdk9-Mo-SB, Cdk-Mo) and Larp7 morpholino (C, Larp7-Mo-SB, Larp7-Mo) on total cardiomyocyte number in the embryonic ventricle (white bars, controls, appropriate mismatch morpholino; dark bars, splice-blocking morpholino. Effects of flavopiridol on the number of proliferating cardiomyocytes (D, white bars, controls; black bars, flavopiridol 3µmol/l). Representative confocal images (E) of isolated embryonic myl7:GFP hearts stained with DAPI (blue) and for phospho-Histone-H3 (PHH3, red nuclei, marking proliferating cardiomyocytes). Data are mean±s.e.m., n=3 experiments, n=5 hearts per group, *P<0.05, **P<0.01 ***P<0.001, two-way ANOVA test. Means±s.e.m. are shown in A, B, C and D.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.