FIGURE

Fig. 11

ID

ZDB-FIG-160205-125

Publication

Bhadra et al., 2015 - Hsp47 mediates Cx43-dependent skeletal growth and patterning in the regenerating fin

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Fig. 11

Disruption of actinotrichia following changes in Hsp47 or Cx43 expression. Single plane confocal sections are obtained for transverse sections immunostained with Collagen type II (green) and counterstained for nuclei with To-pro (blue). (A) Fins are injected with standard control MO, Hsp47-MO or Cx43-MO and evaluated at 1 dpe. Col II expression is disrupted following Hsp47 and Cx43 knockdown. (B) Variable actinotrichia phenotypes observed in 5 dpa transverse sections of both sof ^b123 and alf ^dty86. The defects were categorized as mild, disrupted organization, or small. Arrows indicate actinotrichia, ‘e’ is epidermis and ‘m’ mesenchyme. Scale bar is 10µm and applies to all panels.

Expression Data

Antibody:	Ab1-col2a
Fish:	WT gja1b^b123/b123 kcnk5b^ty86d/ty86d WT + MO1-serpinh1b WT + MO2-gja1b
Condition:	amputation: fin
Knockdown Reagents:	MO1-serpinh1b MO2-gja1b
Anatomical Term:	regenerating fin actinotrichium
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gja1b^b123/b123 kcnk5b^ty86d/ty86d WT + MO1-serpinh1b WT + MO2-gja1b
Condition:	amputation: fin
Knockdown Reagents:	MO1-serpinh1b MO2-gja1b
Observed In:	regenerating fin actinotrichium
Stage:	Adult

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 138 Pt 3, Bhadra, J., Iovine, M.K., Hsp47 mediates Cx43-dependent skeletal growth and patterning in the regenerating fin, 364-74, Copyright (2015) with permission from Elsevier. Full text @ Mech. Dev.