Fig. 5

Morpholino mediated knockdown of Hsp47 causes reduced cell proliferation and fin length. All fins were amputated at the 50% level prior to injection and electroporation. Fin rays treated with targeting or control MO (injected) were measured and compared to their uninjected sides. The ratio of the injected side (targeting MO or control MO) and the control uninjected side, multiplied by 100, is the percent similarity. Percent similarity of greater than 100% reflects the fact that the experimental side can be measurably larger than the control uninjected side. (A–C) Total number of H3P positive cells (white arrows identify one positive cell in each panel) were counted in injected and uninjected sides for Hsp47-MO (A) and Hsp47-5MM (B) treated fins. (C) The bar graph shows reduced level of H3P positive cells in Hsp47 MO injected fins compared to the control MO. (D–F) Total regenerate length in Hsp47-MO (D) and Hsp47-5MM (E) injected fins was measured by evaluating the distance between the distal tip of the fin and the amputation plane (indicated by red arrows) and normalized to the respective uninjected side. (F) Bar graph shows reduced regenerate length in Hsp47 MO treated fins. Scale bar is 50µm and applies to all panels. The student′s t-test was performed (p < 0.05) to determine significance.