FIGURE

Fig. 1

ID

ZDB-FIG-151207-1

Publication

Karra et al., 2015 - Myocardial NF-κB activation is essential for zebrafish heart regeneration

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Fig. 1

NF-_KB activity is induced by injury and during regeneration. (A) qPCR for NF-_KB target genes in regenerating ventricles at 7 dpi in cmlc2:CreER; βact2:RS-DTA animals treated with tamoxifen (n = 6) compared with ventricles from cmlc2:CreER; βact2:RS-DTA animals treated with vehicle (n = 6). Error bars indicate SE. *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t test, two-tailed. Each replicate consists of a pool of 2–3 ventricles. (B–E) In situ hybridization for nfkbiaa or nfkbiab on sections from ventricles of cmlc2:CreER; βact2:RS-DTA animals treated with vehicle (B and D) or tamoxifen (C and E). Violet staining indicates expression. (F–I) Time course of NF-_KB:eGFP induction during regeneration in sections from an uninjured ventricle, a 1 dpa ventricle, a 7 dpa ventricle, and a 14 dpa ventricle. Ventricles are counterstained with an antibody against Tnnt (gray) to delineate cardiomyocytes. (Scale bars: 100 µm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA