ZFIN Image: Karra et al., 2015, Fig. 1

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Figure Caption

Fig. 1

NF-_KB activity is induced by injury and during regeneration. (A) qPCR for NF-_KB target genes in regenerating ventricles at 7 dpi in cmlc2:CreER; βact2:RS-DTA animals treated with tamoxifen (n = 6) compared with ventricles from cmlc2:CreER; βact2:RS-DTA animals treated with vehicle (n = 6). Error bars indicate SE. *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t test, two-tailed. Each replicate consists of a pool of 2–3 ventricles. (B–E) In situ hybridization for nfkbiaa or nfkbiab on sections from ventricles of cmlc2:CreER; βact2:RS-DTA animals treated with vehicle (B and D) or tamoxifen (C and E). Violet staining indicates expression. (F–I) Time course of NF-_KB:eGFP induction during regeneration in sections from an uninjured ventricle, a 1 dpa ventricle, a 7 dpa ventricle, and a 14 dpa ventricle. Ventricles are counterstained with an antibody against Tnnt (gray) to delineate cardiomyocytes. (Scale bars: 100 µm.)

Acknowledgments

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