ZFIN ID: ZDB-FIG-151204-13
Pagán et al., 2015 - Myeloid Growth Factors Promote Resistance to Mycobacterial Infection by Curtailing Granuloma Necrosis through Macrophage Replenishment. Cell Host & Microbe   18:15-26 Full text @ Cell Host Microbe
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 6
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 6

Fig. S2

(Related to Figure 1). Larval csf1r mutant zebrafish have impaired macrophage development. Enumeration of larval macrophages in wild-type (WT) and csf1r-/- mpeg1:YFP fish. (A) Maximum intensity projections of the hindbrains of WT and csf1r-/- mpeg1:YFP larvae at 3dpf. Scale bars, 100µm. Number of macrophages in the (B) midbrain and (C) CHT of 3dpf fish. (D) Absolute numbers of macrophages at 6dpf. Number of neutrophils in the CHT of 3dpf fish as determined by Sudan Black staining. (B - E) Each symbol represents individual larvae. (B - D) Data are representative of at least two experiments. (F - H) Assessment of macrophage motility: (F) Representative maximum intensity projections of the CHT of WT and csf1r-/- mpeg1:YFP larvae at 6dpf and (G) average sphericity and (H) speed of individual macrophage tracks. Hashed boxed indicate zoomed region displayed on right corner. Scale bar, 40µm. Horizontal lines indicate means (B - E) or median values (G, H). Statistical significance determined by two-tailed unpaired Student’s t test (B - E) or Mann-Whitney U test (G, H).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
YFP csf1raj4blue/j4blue; w200Tg control Protruding-mouth hindbrain macrophage IFL
Day 6 macrophage IFL
w200Tg control Protruding-mouth hindbrain macrophage IFL
Day 6 macrophage IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
csf1raj4blue/j4blue; w200Tg control Protruding-mouth blood island macrophage decreased amount, abnormal
Protruding-mouth midbrain macrophage decreased amount, abnormal
Day 6 blood island macrophage decreased amount, abnormal
Day 6 caudal fin macrophage decreased amount, abnormal
Day 6 macrophage decreased amount, abnormal
Day 6 macrophage decreased speed, abnormal
Day 6 macrophage spherical, abnormal
Day 6 midbrain macrophage decreased amount, abnormal
Day 6 pericardium macrophage decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Host & Microbe for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Host Microbe