Fig. 6

Extent of Extrinsic Feedback during Retina Development

(A) Fate distribution of clones generated by WT cells transplanted into Ptf1a, Atoh7, Vsx1 or Atoh7, Ptf1a double morphant hosts. indicates significance compared with WT, while # denotes significant difference between one- and two-cell clones within a particular morphant. Figure legend is also valid for (B). Error bars depict SEM.

(B) Average clone size generated from single RPCs scored at 24 hpf for WT cells and the various environments previously described.

(C–E) Representative micrographs of WT clones generated in Atoh7, Ptf1a, or Vsx1 morphant environments, respectively.

(F) Checker-plot visualizing the extent of intrinsic regulation and extrinsic feedback in the different Atoh7 morphant scenarios. Color denotes deviation from WT to WT, such that a brighter color corresponds to an increase while a darker corresponds to a decrease of cell numbers of a given cell type.

(G and H) The intrinsic model is modified by the introduction of a delay postponing the onset of neurogenesis in 60% of the RPCs by one division. The resulting clone size (G) and fate (H) distributions match the experimental clones. Inset depicts averages of the experimental values and the values from the modeling with corresponding SDs.

(I–K) As in (F)–(H) but for the Ptf1a morphant environment with the same delay introduced to the intrinsic model.

The scale bar in (E) represents 10 µm and is also valid for (C) and (D). See also Figures S5 and S6 and Table S5.